Heavy metal detox studies
Conclusion: Humic and fulvic acids can remove toxic metals from water.
Title: Humic and fulvic acids as reducing agents of potentially toxic metals in water
Document Type: Peer-Reviewed Journal Article
Conclusion: This work presents a highly efficient and relatively low-cost method for reducing the level of potentially toxic metals in water. The reducing agents were humic and fulvic acids isolated from lignite (an abundant and cheap coal) with a simple, rapid and economical procedure. The maximum binding capacity (for a single metal) was presented by fulvic acids in the following order Cr>Pb>Ni>Cu>Cd, while for humic acids it was Pb>Ni>Cr>Cu>Cd.
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Conclusion: Humic substances can protect against viruses, heavy metal toxicity and ionizing radiation.
Title: Medical aspects and applications of humic substances.
Document Type: Peer-Reviewed Journal Article
Conclusion: In addition to the classical use of peat in balneotherapy and veterinary medicine, the application of isolated humic substances (HS) as well as synthetic polymers similar to humic acids (HA) may play an important role in the future. There is a large number of phenolic compounds that can be transformed into HA-like substances intended for special functions, such as antivirally active agents, heavy metal chelating compounds, polymers that bind toxic chemicals and substances protecting against ionizing radiation.
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Conclusion: Humic acids have anti-cancer and anti-aging properties and may help protect against toxicity caused by iron, lead, copper, and mycotoxins.
Title: Therapeutic Efficacy of Humic Acids in Poisoning
Article type: Review article
Abstract: Humic acids (HA) possess antioxidant activity. This property also confers HA its anticancer effect. Furthermore, HA administration may be useful as a supportive treatment alongside cancer chemotherapy, where it protects tissue cells during chemotherapy application and prevents (mitigates) oxidative damage to these cells. The antioxidant effects of HA may also be beneficial in anti-aging therapy, reducing social stress and promoting wound healing, as well as in protecting against iron-induced hepatotoxicity and cardiotoxicity. Furthermore, HA may protect against lead and copper poisoning, mycotoxins, and other toxicants.
Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10143271/
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Conclusion: Humic and fulvic acids may protect the body against oxidative damage.
Title: Humic and fulvic acids alleviate hydrogen peroxide-induced oxidative damage.
Document Type: Peer-Reviewed Journal Article
Conclusion: Both humic acid and fulvic acid showed a significant decrease in the enzymatic activity of glutathione peroxidase (GPx) and superoxide dismutase (SOD), indicating their direct protective effects against induced oxidative stress, through the modulation of the defense enzymes GPx and SOD.
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Conclusion: Humic acid is effective in detoxification, providing some useful nutrients and strengthening the immune system.
Title: Humic Acid: The Power of Detoxification and Immune Support All in One
Document Type: Web Page
Bottom Line: Because humic acid is too large to be absorbed into the bloodstream, redistribution of toxins is not a concern, making it ideal for detoxification. The compound binds substances in the digestive tract to remove them from the body. Additionally, humic acid contains nutrients the body needs and can boost our immune response.
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Conclusion: Humic acid can help in liver regeneration, remove toxic metals and increase the oxygen-carrying capacity of red blood cells.
Title: Effects of humic acid on animals and humans: an overview of the literature and a review of current research
Document Type: Online scientific document
Conclusion: This review article discusses the beneficial properties of humic acids, including their potential role in liver regeneration, detoxification, and improving the oxygen-carrying capacity of red blood cells.
Link: https://www.vetservis.sk/media/object/433/effects_of_humic_acid_on_animals_and_humans.pdf
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Conclusion: Fulvic acid binds heavy metals in dietary sources and prevents their accumulation in the body.
Title: Dietary supplementation of fulvic acid to enhance growth and prevent heavy metal accumulation in Nile tilapia fed with green mussel.
Document Type: Peer-Reviewed Journal Article
Conclusion: The present study demonstrated that dietary supplementation of fulvic acid could be used as a strategy to prevent Pb and Cd accumulation in fish fed a diet containing green-lipped mussel and a supplementation level of 200 mg kg−1 could be recommended to prevent Pb and Cd accumulation and improve growth of Nile tilapia culture.
Link: https://www.sciencedirect.com/science/article/pii/S1687428520300340
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Conclusion: Fulvic acid binds lead and helps prevent lead toxicity.
Title: Lead(II)-fulvic acid complexes. Conditional stability constants, solubility, and implications for lead(II) mobility.
Document Type: Peer-Reviewed Journal Article
Conclusion: Fulvic acid binds lead and helps prevent lead toxicity. This study compares the complexation of lead(II) with fulvic acids derived from podzol soil (SFA) and river water (WFA). In 0.1 M electrolytes, complexation by SFA is stronger than by WFA. From pH 4.0 to 6.0, the logarithm of the lead(II)-SFA 1:l conditional stability constants increases from 4.0 to 6.0. 0 to 6.3. The corresponding constants for lead(II)-WFA in the pH range 4.5 to 6.0 increase from 3.7 to 5.1. Lead(II) forms insoluble precipitates in proportions low lead(II)/fulvic acid molars.
Link: https://sci-hub.se/https://pubs.acs.org/doi/pdf/10.1021/es60167a001
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Conclusion: Humic acid binds heavy metals and can improve the utilization of heavy metal-containing feeds for fish growth.
Title: Response to the addition of humic acid to feed containing heavy metals made from green mussels on the growth of Asian sea bass
Document Type: Peer-Reviewed Journal Article
Conclusion: The addition of humic acid (HA) in animal feed brings many advantages, one of which is that it can chelate heavy metals. The addition of HA in feed formulation is necessary to prevent the accumulation of heavy metals in the body of fish. An experiment was designed to evaluate the response of HA addition in diets made from green mussels containing heavy metals on the growth performance and feed efficiency of juvenile Asian seabass, Lates calcarifer. A total of five experimental diets were formulated with different HA addition doses (0, 400, 800, 1200, 40, and 1600) mg Kg-1 of feed in the experimental diets (A, B, C, D, and E), respectively. The experimental diets were fed in triplicate to a group of 15 juvenile Asian seabass with an initial body weight of 4.30 ± 0.60 g in a 70-day culture by feeding to satiation. The result shows that feed intake, feed digestibility, protein retention, growth performance and feed efficiency were significantly affected by the addition of HA in fish diet (P<0.05). Treatment E with HA addition with a diet of 1600 mg Kg-1 was shown to give the best biological response among all treatments. This indicates that the addition of humic acid in diet formulation can improve feed utilization for fish growth. The heavy metal Cd in Asian seabass meat is still below the limit set in the food safety standard, so the fish in this experiment is still considered safe for human consumption. The present study concluded that green mussel meal as an alternative protein source with HA addition can be considered as a potential feed additive for the diets of Asian seabass juveniles.
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Conclusion: Fulvic acid helps treat a variety of diseases and protects the body against the accumulation of heavy metals.
Title: Titrimetric estimation of fulvic acid substances in Oriens Shilajit as part of standardization of herbal nutraceuticals
Document Type: Peer-Reviewed Journal Article
Conclusion: Fulvic acids are a family of organic acids and form a major part of the organic matter content of the soil. In the present study, we aimed to estimate the amount of fulvic acids in Shilajit, a well-known medicinal application in Ayurveda by titration method. We obtained a fulvic acid percentage of 0.96% (w/w). Fulvic acid in Shilajit can be used to treat a variety of ailments and also to protect the body against heavy metal accumulation.
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Conclusion: Humic acid binds to chromium and thus reduces its toxicity.
Title: The effect of humic acid on the toxicity and bioavailability of trivalent chromium.
Document Type: Peer-Reviewed Journal Article
Conclusion: The influence of humic acid (HA) on the toxicity and bioavailability of two forms of trivalent chromium (chromic chloride and chromium lignosulfonate) was evaluated using a common freshwater invertebrate, Daphnia pulex. For both compounds, the dose of 50 mg/liter of HA significantly decreased toxicity at all time points examined. The two remaining concentrations of HA, 0.5 and 5 mg/liter, had no influence or decreased the toxicity of the compounds. Humic acid did not appear to have an influence on the bioavailability of chromium lignosulfonate. However, for chromic chloride, 5 and 50 mg/liter of HA decreased the percentage of free chromium at all time points examined.
Link: https://sci-hub.se/10.1016/0147-6513(89)90014-6
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Conclusion: Fulvic acid reduces cadmium accumulation, thus reducing cadmium toxicity.
Title: Role of fulvic acid in reducing cadmium toxicity in tilapia (Oreochromis niloticus)
Document Type: Peer-Reviewed Journal Article
Conclusion: The effect of fulvic acid on cadmium (Cd) toxicity, impact on immunological and hematological changes of fish in Nile tilapia (Oreochromius niloticus) were studied. Fish (100±10g) were exposed to 10 ppm Cd alone or with 0.1, 0.2 and 0.3 ppm for 15 and 45 days. Cd exposure was significantly reduced (P<0.04), for example, red blood cell (RBC) count, hemoglobin (Hb) content, hematocrit (Hct) value, mean cell hemoglobin (MCH) and mean cell hemoglobin concentration. These parameters were improved when fulvic acid was applied with Cd. RBC, Hb, Hct, MCH and MCHC values were significantly increased in the control group of fish. The addition of fulvic acid to Cd-contaminated medium significantly reduced metal uptake and accumulation in fish tissues, whereas metals in water and feces increased. Fish exposed to Cd alone accumulated 2.15 and 5.970 mg Cd/g dry weight in liver tissue for 15 and 45 days, respectively. Cd was significantly reduced to 1.292 and 4.16; 0.92 and 3.791; and 0.41 and 2.43 mg Cd/g dry weight of tissue in fish exposed to 0.1, 0.2, and 0.3 g fulvic acid/L for 15 and 45 days, respectively. Similar trends were observed in gills and musculature.
Link: https://www.witpress.com/elibrary/wit-transactions-on-ecology-and-the-environment/132/20937
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Conclusion: Humic acid reduces heavy metal accumulation in fish and protects against toxicity-related death.
Title: Effect of humic acid during simultaneous chronic exposure of rainbow trout (Oncorhynchus mykiss) to copper, cadmium and zinc via water
Document Type: Peer-Reviewed Journal Article
Conclusion: Commercial dissolved organic carbon (DOC), in the form of humic acid, at an environmentally relevant concentration reduced the bioavailable fractions of metals (especially Cu), leading to reduced accumulation, alleviating biochemical alterations and protecting against mortality.
Link: https://sci-hub.se/https://pubmed.ncbi.nlm.nih.gov/20970854/
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Conclusion: Humic acid reduces cadmium toxicity and accumulation.
Title: Effects of humic acid and water hardness on acute toxicity and cadmium accumulation in freshwater fish (Puntius gonionotus Bleeker)
Document Type: Peer-Reviewed Journal Article
Conclusion: An increase in humic acid concentrations (0.5, 5.0, and 50 mg/L) decreased cadmium toxicity and accumulation in the freshwater fish Puntius gonionotus Bleeker. Furthermore, simultaneous increases in humic acid and water hardness concentrations also resulted in decreased cadmium toxicity.
Link: https://thaiscience.info/Journals/Article/SCAS/10460003.pdf
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Conclusion: Humic acid at adequate concentrations reduces cadmium toxicity.
Title: The influence of humic acid and the toxicity and bioavailability of selected trace metals.
Document Type: Peer-Reviewed Journal Article
Conclusion: The influence of humic acid (HA) on the toxicity and bioavailability of hexavalent chromium and cadmium was assessed using a common freshwater invertebrate, Daphnia pulex. Concentrations of 0.5, 5.0, and 50 mg HA/L had little influence on either the acute toxicity or bioavailability of hexavalent chromium. Acute cadmium toxicity was either increased, decreased, or not influenced by HA, depending on the HA concentration and time point examined. A dose-related binding pattern (a maximal decrease in percent free cadmium followed by metal release from HA) was observed with HA and cadmium. Cadmium release from HA may have resulted from an associated time-dependent decrease in pH.
Link: https://sci-hub.se/https://www.sciencedirect.com/science/article/abs/pii/0166445X88900367
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Conclusion: Humic acid decreases copper toxicity in some organisms.
Title: Toxicity and bioaccumulation of cadmium and copper affected by humic acid.
Document Type: Peer-Reviewed Journal Article
Conclusion: The toxicity of cadmium (Cd) and copper (Cu) to daphnids was evaluated during a 42-day exposure period to three concentrations of humic acid. The addition of humic acid to the test water decreased the acute and chronic toxicity of Cu but increased the acute and chronic toxicity of Cd.
Link: https://sci-hub.se/https://www.sciencedirect.com/science/article/abs/pii/0166445X84900250
